Abstract
Transgenic hairy root system is a promising source of secondary metabolites in medicinal plants with high pharmaceutical value.For the first time, hairy roots were established in different explants of Linum mucronatum, an anti-cancer agent producing plant, via amikimopine type strain of Agrobacterium rhizogenes, ‘A13’. The percentage of hairy root induction varied from 0 to 60% depended onthe explants and hypocotyl (including cotyledonary node) explants were found to be highly susceptible to A. rhizogenes infection withthe highest (60%) rate of hairy root induction. four different Murashige and Skoog (MS)-based liquid culture media were used for wellestablishment of hairy roots. Hairy root growth medium D (HRGM-D) containing hormone-free MS basal medium with an extra oneday pre-incubation period at 35°C was found to be more efficient for profuse growth (fresh weight; 8500 mg per 25 ml culture medium)of hairy roots. Hairy root system presented in this study may offer a suitable platform for optimization and production of satisfactorylevel of aryltetralin lignans like podophyllotoxin and its derivatives from L. mucronatum.
Highlights
Linum spp. from section Syllinum are promising for the production of aryltetralin lignans like podophyllotoxin (PTOX) and 6-methoxypodophyllotoxin (6-MPTOX) (Mohagheghzadeh et al, 2007)
Hypocotyl explants were highly susceptible to infection by strain ‘A13’ of A. rhizogenes, as shown by the percentage (60%) of them from which hairy roots emerged, whereas; root explants exhibited the lowest infection frequency (20%)
The results indicated that wild type strain of A. rhizogenes used in this study were able to impressively induce hairy roots on hypocotyl explants that can be used for mass production of hairy roots in L. mucronatum
Summary
Linum spp. from section Syllinum are promising for the production of aryltetralin lignans like podophyllotoxin (PTOX) and 6-methoxypodophyllotoxin (6-MPTOX) (Mohagheghzadeh et al, 2007). PTOX is the main lignan in the cell cultures of Linum album and 6-MPTOX is predominantly accumulated in cell lines of L. flavum, L. nodiflorum, L. mucronatum, and L. tauricum. This lignan and its derivatives possess anti-cancer properties (Ionkova, 2007). The greatest advantage of hairy roots is that their cultures often exhibit approximately the same or greater biosynthetic capacity for secondary metabolite production as compared to their mother plants (Kim et al, 2002) They can be a promising source for the continuous and standardized production of secondary metabolites under controlled conditions without losing genetic or biosynthetic stability (Giri and Narasu, 2000). Successful induction of hairy root has been reported in some Linum spp. such as L. flavum (Lin et al, 2003; Oostdam et al, 1993), L. austriacum (Mohagheghzadeh et al, 2002), L. leonii (Vasilev et al, 2006) and L. tauricum (Ionkova and Fuss, 2009) and main metabolites (Lignans) from resulted hairy roots have been shown to have biological activity
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