Abstract
A cell-free amino acid incorporating system has been prepared from mammalian hair root cells obtained by epilating animals with latex paint. The system requires a particulate fraction from the tissue homogenate (microsomes or ribosomes), a supernatant fraction in the case of ribosomes, ATP, GTP and magnesium. The particulate fraction is composed primarily of monomeric ribosomes which are as synthetically active as is the small amount of material with a sedimentation coefficient corresponding to polymeric ribosomes. Following in vivo injection of radioactively labeled amino acids, newly synthesized proteins are predominantly associated with the monomeric ribosomes. Ribonuclease has been identified in the components of the cell-free system whose activity is lower than that of a similar system prepared from mammalian liver.
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