Abstract

Alopecia is a chronic inflammatory skin disease with various causes. Lespedeza bicolor extract (LBE) has been reported to have anti-inflammatory and antioxidative effects. In this study, the activity and mechanisms of LBE as a hair growth agent were investigated. Effects of cell proliferation, cytotoxicity, and cell cycle regulation of LBE and its active component protocatechuic acid (PCA) were evaluated in human dermal papilla cells (DPCs). Hair regeneration effects of LBE in 6-week-old C57BL/6 male mice were also determined using positive control 5% minoxidil. The dose-dependent proliferation of DPCs was estimated in response to LBE treatment (0.8–20 µg/mL). Additionally, significant extension of the anagen phase during the hair cell cycle upon LBE treatment was observed histologically and morphologically. Cell cycle arrest gene expression was determined by quantitative real-time polymerase chain reaction. Lespedezabicolor could be a potent treatment against alopecia through enhancing DPC proliferation and hair regrowth via anagen phase arrest.

Highlights

  • Alopecia, known as hair loss, is a dermatological disorder with a global distribution

  • Because hair follicles (HF) are composed of dermal papilla cells (DPCs) and skin sheaths of human hair, they have an essential function of controlling hair growth throughout pathogenesis of specific conditions, including hair loss, as well as during the normal hair cycle [5]

  • Cytotoxicity was observed in DPCs treated with high concentrations of Lespedeza bicolor extract (LBE), this treatment did not induce any morphological changes

Read more

Summary

Introduction

Known as hair loss, is a dermatological disorder with a global distribution. The hair growth cycle moves through several phases, including degeneration (catagen phase), rest (telogen phase), and regeneration (anagen phase) in adult hair follicles (HF) for extended periods [1–4]. Because HFs are composed of dermal papilla cells (DPCs) and skin sheaths of human hair, they have an essential function of controlling hair growth throughout pathogenesis of specific conditions, including hair loss, as well as during the normal hair cycle [5]. Hair loss is often caused by a chronic scalp inflammation reaction and, as a result, the anagen phase of HF is shortened [7], and all or part of the hair of the head or body may be lost [8]. The effects of LBE on DPC proliferation and cell cycle were investigated. Hair regeneration promotion effects by LBE were observed in C57BL/6 mice

Methods
Results
Discussion
Conclusion
Full Text
Paper version not known

Talk to us

Join us for a 30 min session where you can share your feedback and ask us any queries you have

Schedule a call

Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.