Abstract
The successful development of safe and highly effective nanoprobes for targeted imaging and simultaneous therapy of in vivo gastric cancer is a great challenge. Herein we reported for the first time that anti-α-subunit of ATP synthase antibody, HAI-178 monoclonal antibody-conjugated fluorescent magnetic nanoparticles, was successfully used for targeted imaging and simultaneous therapy of in vivo gastric cancer. A total of 172 specimens of gastric cancer tissues were collected, and the expression of α-subunit of ATP synthase in gastric cancer tissues was investigated by immunohistochemistry method. Fluorescent magnetic nanoparticles were prepared and conjugated with HAI-178 monoclonal antibody, and the resultant HAI-178 antibody-conjugated fluorescent magnetic nanoparticles (HAI-178-FMNPs) were co-incubated with gastric cancer MGC803 cells and gastric mucous GES-1 cells. Gastric cancer-bearing nude mice models were established, were injected with prepared HAI-178-FMNPs via tail vein, and were imaged by magnetic resonance imaging and small animal fluorescent imaging system. The results showed that the α-subunit of ATP synthase exhibited high expression in 94.7% of the gastric cancer tissues. The prepared HAI-178-FMNPs could target actively MGC803 cells, realized fluorescent imaging and magnetic resonance imaging of in vivo gastric cancer, and actively inhibited growth of gastric cancer cells. In conclusion, HAI-178 antibody-conjugated fluorescent magnetic nanoparticles have a great potential in applications such as targeted imaging and simultaneous therapy of in vivo early gastric cancer cells in the near future.
Highlights
Gastric cancer is the second most common cancer and the third leading cause of cancer-related death in China [1,2,3]
Expression of α-subunit of ATP synthase in gastric cancer tissues Figure 1A showed the positive expression of α-subunit of ATP synthase in gastric cancer tissues; Figure 1B showed the negative expression of α-subunit of ATP synthase in gastric mucous tissues
We investigated the expression of α-subunit of ATP synthase in 172 specimens of gastric cancer tissues by immunohistochemistry method
Summary
Gastric cancer is the second most common cancer and the third leading cause of cancer-related death in China [1,2,3]. How to recognize and track or kill early gastric cancer cells is a great challenge for early diagnosis and therapy of patients with gastric cancer. We have tried to establish an early gastric cancer prewarning and diagnosis system since 2005 [5,6]. We hoped to find early gastric cancer cells in vivo by multi-mode targeting imaging and serum biomarker detection techniques [7,8,9,10,11,12]. The clinical translation of these prepared nanoprobes still exists as a great challenge because no one kind of biomarker is specific for gastric cancer. Looking for new potential biomarker of gastric cancer and development of safe and effective nanoprobes for targeted imaging and simultaneous therapy of in vivo early gastric cancer have become our concerns
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
