Haemonchus contortus excretory and secretory proteins (HcESPs) suppress functions of goat PBMCs in vitro.

Javaid Ali Gadahi,Ruo Feng Yan,Muhammad Ehsan,Xiang Rui Li,Bu Yongqian,Xiao Kai Song,Li Xin Xu,Shuai Wang,Zhen Chao Zhang

doi:10.18632/oncotarget.9589

Javaid Ali Gadahi, Ruo Feng Yan + Show 7 more

Open Access

https://doi.org/10.18632/oncotarget.9589

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Journal: Oncotarget	Publication Date: May 25, 2016
Citations: 88	License type: cc-by

Affiliation: Nanjing Agricultural University

Abstract

Excretory and secretory products (ESPs) of nematode contain various proteins which are capable of inducing the instigation or depression of the host immune response and are involved in the pathogenesis of the worms. In the present study, Haemonchus contortus excretory and secretory products (HcESPs) were collected from the adult worms. Binding of HcESPs to goat peripheral blood mononuclear cells (PBMCs) was confirmed by immune-fluorescence assay. Effects of the HcESPs on cytokine production, cell proliferation, cell migration and nitric oxide (NO) production of PBMCs were checked by co-incubation of HcESPs with goat PBMCs. The results indicated that the production of IL-4 and IFN-γ were significantly decreased by HcESPs in dose dependent manner. On the contrary, the production of IL-10 and IL-17 were increased. Cell migration was significantly enhanced by HcESPs, whereas, HcESPs treatment significantly suppressed the cell proliferation and NO production. These results indicated that the HcESPs played important suppressive regulatory roles on PBMCs and provided highlights to the understanding of the host-parasite interactions.

Highlights

Gastrointestinal nematodes generally develop chronic infections and survive themselves in the host for a longer duration
We evaluated the immune-modulatory effects of Haemonchus contortus excretory and secretory products (HcESPs) on the goat peripheral blood mononuclear cells (PBMCs)
Goat PBMCs were incubated with HcESPs and the binding was investigated by IFA

Summary

INTRODUCTION

Gastrointestinal nematodes generally develop chronic infections and survive themselves in the host for a longer duration. The purified 66-kDa adult H. contortus excretory/ secretory (E/S) antigen was confirmed to suppress monocyte function in vitro by decreasing the production of hydrogen peroxide and nitric oxide in the culture medium of the cells [3]. In vitro studies showed that ESPs had direct effects on cultured cells or tissues, such as inhibiting acid secretion [19] and inducing the vacuolation and detachment of HeLa cells [20, 21]. These findings indicated that ESPs might play multiple functions in vivo. We evaluated the immune-modulatory effects of HcESPs on the goat PBMCs

RESULTS

DISCUSSION

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