Abstract
The ability of subcellular fractions of rabbit spleen and liver to induce haemolysis of rabbit erythrocytes was investigated. It was shown that: Subcellular fractions of normal spleen and liver induce haemolysis of rabbit erythrocytes modified in vivo by injections of small doses of phenylhydrazine. The haemolytic activity of both tissue preparations was located mainly in the mitochondria and was much higher in the spleen than in the liver. Fresh preparations of the spleen or the liver do not induce haemolysis of intact erythrocytes. When the tissue fractions and also the erythrocytes were obtained from phenyl‐hydrazine treated rabbits, the haemolytic activity of the spleen preparations was lower and that of the liver was slightly higher than the respective activities of preparations of normal tissues. The haemolytic activity of the subcellular fractions kept frozen for 7 days at pH = 7.0 was significantly increased. Phospholipase A is indicated as a possible haemolytic agent present in the spleen and the liver mitochondria.
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