Abstract
The use of photodynamic antimicrobial chemotherapy (PACT) to treat localized Candida infections is an emerging and promising therapeutic approach. To assess the photodynamic inactivation (PDI) efficacy of haematoporphyrin monomethyl ether (HMME) on the biofilms produced by standard (ATCC 10231) and fluconazole-resistant Candida albicans strains. The PDI efficacy was evaluated by incubating C. albicans biofilms with 0.01-10 μmol/L HMME for 30 min in the dark, followed by irradiation with 400-800 nm light for 30 min. HMME inactivated the biofilms in a concentration-dependent manner in the presence of light, but it exhibited no significant toxicity without light. After treatment with 10 μmol/L HMME and irradiation for 30 min, 2.47 and 3.26 log10 reductions in survival were achieved for C. albicans ATCC 10231 and fluconazole-resistant C. albicans, respectively. Biofilms formed by the two strains were sensitive to HMME-mediated PDI.
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