Abstract
The effect of sub lethal concentrations of fresh root bark extracts of Plumbago zeylanica on blood compositions of Clarias gariepinus was investigated under static water renewal bioassay during a 21-day exposure period. Concentrations of Plumbago zeylanica extracts used were 0 (control), 26, 39 and 59 mgl-1. The exposure led to anaemic response in fish, this was observed to be time and dose dependent. There were significant decreases in haematological values of PCV (20.66 ± 2.84), Haemoglobin (6.73 ± 0.87), RBC (1.70 ± 0.02), MCV (32.67 ± 0.33), MCHC (38.67 ± 4.33), and MCH (119.67 ± 14.84) at 59 mgl-1. While slight increase was observed in WBC (16016.67 ± 1717. 63) at 39 mgl-1. The muscle/flesh of the exposed fish showed goblet cell hyperplasia, muscle necrosis and calcification at lower concentrations of exposure for both the short term and long term exposure, but disappears at higher concentrations after one week withdrawal without bioaccumulation in the Organs and Tissues. The changes observed indicates that haematological indices could be used as risk assessment tools in aquatic environmental monitoring to assess physiological status of fish exposed to sub lethal effect of toxicants.
Highlights
The use of haematological techniques in fish culture is of growing importance to toxicological research, environmental monitoring and fish health conditions
The paper present findings of an investigation on the haematological response and biochemical parameters of Clarias gariepinus exposed to chronic sublethal concentrations of fresh root bark cold water extract (FRBCWE) of Plumbago zeylanica under laboratory conditions
The result shows a decrease in the Mean Red blood cell (RBC), Packed cell volume (PCV), and Haemoglobin compared to the initial values
Summary
The use of haematological techniques in fish culture is of growing importance to toxicological research, environmental monitoring and fish health conditions. Blood is a good indicator to determine the health of an organism It act as a pathological reflector of the whole body, haematological parameters are important in diagnosing the functional status of animals exposed to toxicants. Application of synthetic pesticides is one of the methods used to control fish population Due to their long-term persistence, slow degradability in water, toxicity to other organisms [1] and accumulation inside the fish body, synthetic piscicides adversely affect the aquatic environment [2]. To solve this problem, studies have been carried out on the possibility of using plants as piscicides [3] and they are considered safe for users. The paper present findings of an investigation on the haematological response and biochemical parameters of Clarias gariepinus exposed to chronic sublethal concentrations of fresh root bark cold water extract (FRBCWE) of Plumbago zeylanica under laboratory conditions
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
