Haematological Profile of Rats Administered With Aqueous Leaf Extract of Moringa Oleifera Following Thermooxidixed Palm Oil Diet Induced Toxicity

Abstract

In assessing the effect of haematological parameters of rats administered with aqueous leaf extract of Moringa oleifera following thermooxidixed palm oil induced toxicity. Twenty-five (25) Female rats weighing 150-170g body weight were assigned randomly into five groups: A-E of 5 rats, after permission was granted by the Collage Ethical Committee on Experimental Animals. Groups A serves as control received rat chow and water, Group B was fed with fresh palm oil diet (Diet I), Group C was fed with thermoxidized palm oil diet (diet II), Group D was fed with fresh palm oil diet and 600mg/kg body weight aqueous Moringa oleifera leaf orally for 28days, Group E received thermoxidized palm oil diet and 600mg/kg body weight orally for 28days. They were allowed free access to water and standard feeds ad libitum throughout the experimental periods. The animals were housed in stainless cages at temperature of 28±20 o Cand 12hrs dark cycle and humidity of 60%. After 28 th day of feeding, the rats were allowed an overnight fast. They were anaesthetized with chloroform/diethylether mixture, their thorax was opened and blood was withdrawn by cardiac puncture with sterilesyringeinto EDTA sample bottle for haematological studies.Sequel to this, the bioactive component of Mo ringa oleifera was determined using standard methods.Results shows the presence of alkaloids,glycosides, sapo nins, tannins, flavonoids, reducing compounds, polyphenol, titerpenes, steroid with highest concentration of flav onoids and polyphenols while anthraquinones, hydroxyl methyl and phlobatannins are notdetected.Hematologic al result from RBCs showed significant increase (P<0.001) in FPO, FPO+MO and TPO+MO; WBC were significantly increased in TPO, FPO+MO and TPO+MO. Platelets, HB and HCT were significantly reduced in TPO but were significantly (P<0.001) higher in FPO+MO and TPO+MO. MCV and MCHC were significantly (P<0.001, P<0.05) increase in test groups compared with the control. The granulocyte % were significantly reduced (P<0.001) in TPO and FPO+MO but were increased in FPO while monocyte were significantly reduced in test groups compared with the control. Lymphocytes were significantly (P<0.05) increased in test groups compared with control. The results are indications of its haematopoietic and erythropoietic effect which suggests that the bioactive component of Moringa oleifera might possess a chelative or scarvengive effect on the TPO diet.