Abstract
Haematological and histopathological parameters have been recognized as valuable tools for monitoring fish health. This study was conducted to evaluate the chronic toxicity of Acid Red 97 textile dyes on haematological and histopathological alterations using Catla catla as animal model. Fish were exposed to two Sublethal concentrations (1/100th and 1/50th of LC50 = 85mg/l) 0.85 mg/l and 1.7 mg/l of AR 97 for a period of (10, 20 and 30) days. Haematological parameters were observed that with increase of exposure time, total erythrocyte (RBC), haemoglobin (Hb), and packed cell volume (PCV) values decreased but leucocytes (WBC), mean corpuscular hemoglobin concentration (MCHC), mean corpuscular volume (MCV) and mean corpuscular hemoglobin (MCH) values increased. It is believed that observed depression in packed cell volume and haemoglobin values coupled with decreased and deformed erythrocytes are obvious signs of anemia. Resulted changes in erythrocytes and leukocytes after exposing to AR 97 are due to malfunction in hemopoiesis and decrease in non-specific immune system. Histopathological changes observed in the gills of Catla catla were swelling, aneurysm, fusion of lamellae, oedema in primary lamellae, shortened and severe erosions of secondary lamella and lifting epithelial layer and high mucus secretion. Hence, it was concluded that azo dye AR 97 has potential to cause toxicity in fish.
Highlights
Most of the azo dyes are released into the environmentAR 97 was purchased from local market and used directly for the experiment
Haematological indices like packed cell volume (PCV), mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH) and mean corpuscular hemoglobin concentration (MCHC) were calculated from obtained results of haemoglobin and RBC value of blood samples collected from control as well as exposed fingerlings
It clearly indicates the toxic potential of AR 97 even at sublethal concentrations that affect the haemato-physiology of exposed fingerlings
Summary
Catla catla fingerlings (13±1cm long and 25±2.5g weight) were procured from fish seed farm sivan, surat, Gujarat and acclimatized in the Advances in Zoology and Botany 8(4): 342-350, 2020 laboratory condition for 15 days according to APHA [15]. Apparently healthy catla fingerlings were grouped in to 4 (10 in each) and used to carry out toxicity test. Among this one was considered as control and other 3 groups of catla fingerlings were exposed to sublethal concentration 0.85 and 1.7 mg/l (1/100th and 1/50th of LC50 = 85mg/l) of AR 97 for 30 days in triplicates. At fixed interval (10, 20, 30 days) after the exposure blood samples were collected and were analyzed
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
