Abstract
Haematobiochemical changes of ovine (sheep) blood were investigated during preservation and storage with Citrate Phosphate Dextrose Adenine-1 (CPDA-1) and Acid Citrate Dextrose (ACD) for transfusion. Twelve healthy sheep were selected and divided into two equal groups: group X (n=6) and group Y (n=6). Thirty-five ml of blood was collected from each animal and preserved with CPDA-1 in group X and ACD in group Y under 4°C in refrigerator for 28 days. Haematological changes viz., total erythrocyte count (TEC), total leukocyte count (TLC), haemoglobin (Hb), packed cell volume (PCV), mean corpuscular volume (MCV), mean corpuscular haemoglobin (MCH) and mean corpuscular haemoglobin concentration (MCHC); and biochemical changes viz., total protein (TP) and pH were evaluated immediately after blood collection and thereafter on day-1, day-3, day-7, day-14, day-21 and day-28 for both groups. In ACD preserved blood; TEC, TLC, Hb and PCV decreased significantly (P<0.01) from day-14 onward, whereas in CPDA-1 preserved blood, these parameters decreased significantly (P<0.01) from day-21 onward. Blood preserved in ACD showed significant changes (P<0.01) in MCV, MCH and MCHC respectively from day- 7, day-14 and day-21 onward, whereas blood preserved in CPDA-1 showed no significant changes in the same parameters throughout the experiment. In both groups, no significant changes were noticed in TP but significant changes (P<0.01) were observed in pH with the progression of storage period. These findings elicited that both ACD and CPDA-1 exert certain haematobiochemical changes in stored sheep blood, however, CPDA-1 was more efficient than ACD in terms of maintaining proper levels of TEC, TLC, Hb., PCV, MCV, MCH and MCHC during preservation and storage of sheep blood for transfusion.
 Res. Agric., Livest. Fish.7(1): 113-120, April 2020
Highlights
Blood transfusion has been used as a life-saving therapeutic procedure in veterinary medicine for a long time since Richard Lower first experimented this technique in animal species in 1665 (Davidow, 2013; Kumar, 2017)
Blood samples were collected from the animals in both groups and afterwards, preserved with Citrate Phosphate Dextrose Adenine-1 (CPDA-1) solution in case of group X (n=6); whereas for group Y (n=6), blood samples were preserved with Acid Citrate Dextrose (ACD) solution for further transfusion
The effects of CPDA-1 and ACD on total erythrocyte count (TEC), total leukocyte count (TLC), Hb and packed cell volume (PCV) are presented in Figure 1 and Figure 2, respectively
Summary
Blood transfusion has been used as a life-saving therapeutic procedure in veterinary medicine for a long time since Richard Lower first experimented this technique in animal species in 1665 (Davidow, 2013; Kumar, 2017). Blood cells undergo biochemical, structural, enzymatic, morphological and functional deterioration due to loss of 2,3 diphosphoglycerate (DPG); depletion of ATP; reduction in Na+–K+ gradients; increase in osmotic fragility and membrane changes including micro vesiculation and haemolysis (Hess, 2006; Dzik, 2008). Many of these changes are reversible following transfusion, it is essential to find out the limit of red blood cell (RBC) storage beyond which transfusion is unfavorable. They do not lose their antigenic property and are capable of sensitizing the recipient to produce non-haemolytic febrile transfusion reactions, and few lymphocytes may remain viable even after 3 weeks of storage (Batham and Nayak, 2018)
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