HAEMAGLUTINATION OF Shigella flexneri SUBUNIT PILI PROTEIN 18 KDA AS A MOLECULE ADHESION IN MICE ENTEROCYTES

Abstract

<p><em>Diarrhoeal diseases are still a major cause of the high morbidity and mortality of children globally, especially in children under five years.</em><em> </em><em>Diarrhea caused by </em><em>S</em><em>higella flexneri usual dealt with antibiotics seen it is getting resistant with various antibiotic</em><em>. </em><em> Prevention shigellosis with a vaccine that was developed and used now are still limited</em><em>. T</em><em>his study attempts to explore hemaglutinin protein pili </em><em>S</em><em> . </em><em>f</em><em>lexneri allegedly role in adhesion bacteria on the mice enterocyte.</em><em> </em><em>This research</em><em> using </em><em> hemaglutina</em><em>tion</em><em> </em><em>test </em><em>then adhesion</em><em> test </em><em> by means of </em><em>reacting </em><em>protein </em><em>sub unit </em><em>pili </em><em>18 kDa S. flexneri </em><em>with </em><em>mice </em><em>entero</em><em>cyte cells</em><em> and observed the amount of bacteria inherent in a </em><em>mice </em><em>cell entero</em><em>cyte</em><em>. The research found that</em><em> </em><em>proteins sub unit pili with molecules</em><em> weight</em><em> 18 k</em><em>D</em><em>a is a protein </em><em>that </em><em>capable </em><em>to agglutinate mice </em><em>red blood cells </em><em>with highest</em><em> dilution and capable </em><em>to</em><em> inhibit </em><em>adhesion</em><em> bacteria on the </em><em>mice </em><em>entero</em><em>cyte</em><em> significantly</em><em>.</em></p><p><strong><em> </em></strong></p><p><strong>Keywords</strong>:<em> Shigella flexneri, </em><em>protein </em><em>sub</em><em> </em><em>unit </em><em>pili </em><em>, hemagglutinin protein, adhesin</em><em> protein</em><em>.</em></p>