Habituation in Stentor: produced by mechanoreceptor channel modification

Abstract

Habituation in Stentor was shown in the previous paper (Wood, 1988) to result from a progressive diminution in mechanoreceptor potential amplitude during the course of repetitive mechanical stimulation. The source of this diminution was analyzed in the present studies by eliciting mechanoreceptor currents from control and habituated cells under voltage clamp control. The I-V plots obtained have a voltage-dependent region between -60 and -20 mV, and a linear voltage-independent region between -20 and 20 mV, with a reversal potential near 20 mV. The slope of the linear region did not change as a result of habituation indicating that the maximum mechanoreceptor current, and hence the total number of mechanoreceptor channels, was the same in control and habituated cells. The mechanoreceptor current reversal potential was changed by only a few millivolts, from 21.6 to 17.2 mV, leaving the driving force term for the mechanoreceptor current at resting potential relatively unchanged. On the other hand, the voltage-dependent region of the I-V plot became significantly steeper after habituation (9.6 mV/e-fold change) compared to its value before habituation (12.3 mV/e-fold change). This shift in voltage dependence is sufficient to explain the diminution in receptor potential amplitude seen during normal recordings at resting potential. The importance of the voltage-dependent characteristic of the mechanoreceptor channels to the habituation process was corroborated by the observation that the amplitude of receptor potentials elicited from cells depolarized by current pulses habituated to only a very limited degree while receptor potentials elicited at resting potential underwent marked habituation.(ABSTRACT TRUNCATED AT 250 WORDS)