Abstract
The recombinant antibody H90-10 detects the endogenous human heat-shock protein 90 beta (Hsp90β) by immunoprecipitation (IP) and Western blotting.
Highlights
The mouse monoclonal antibody H90-10 recognizes the Hsp90b (UniProt #P08238) isoform of the Hsp90 family (Holt et al, 1999; Barent et al, 1998)
Medecine/antibodies) as a mini-antibody with the antigenbinding scFv fused to the Fc region of mouse IgG2a
Supernatant (~90 mg/HLs)p90β was collected after 5 days. 35 kDa
Summary
The mouse monoclonal antibody H90-10 recognizes the Hsp90b (UniProt #P08238) isoform of the Hsp90 family (Holt et al, 1999; Barent et al, 1998). Nomenclature, https://web.expasy.org/abcd) was produced by the Geneva Antibody Facility Medecine/antibodies) as a mini-antibody with the antigenbinding scFv fused to the Fc region of mouse IgG2a. The synthesized scFv sequence (GeneArt, Invitrogen) corresponds to the sequence of the variable regions of the anti-Hsp90b monoclonal H90-10 (Holt et al, 1999; Barent et al, 1998) joined by a peptide linker (GGGGS)3.
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