Abstract
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Highlights
RNA interference (RNAi) responses are inherited in Caenorhabditis elegans nematodes across generations via heritable small RNAs (Alcazar et al, 2008; Buckley et al, 2012; Vastenhouw et al, 2006)
Our previous results regarding the role of H3K9me1/2 did not rule out the possibility that H3K9me3 is yet required for efficient heritable silencing of gfp transgenes: We found that RNAi responses in met-2 mutants lead to marking of the target gene’s histones with a heritable H3K9me3 modification
We successfully replicated the results of Kalinava et al, and came to the same conclusion, that the met-2;set-25;set-32 triple mutant worms inherit RNAi responses against the oma-1 gene, when we used a different assay for inheritance (Figure 1A and Figure 1B, upper panel)
Summary
RNA interference (RNAi) responses are inherited in Caenorhabditis elegans nematodes across generations via heritable small RNAs (Alcazar et al, 2008; Buckley et al, 2012; Vastenhouw et al, 2006). Exposure to a number of environmental challenges, such as viral infection (Gammon et al, 2017; Rechavi et al, 2011), starvation (Rechavi et al, 2014), heat (Klosin et al, 2017), and growth in liquid (Lev et al, 2018) induces heritable physiological responses that persist for multiple generations. Inheritance of such transmitted information was linked to inheritance of small RNAs and chromatin modifications, and hypothesized to protect and prepare the progeny for the environmental challenges that the ancestors met. In C. elegans small RNAs that enter the nucleus were shown to inhibit the elongation phase of Pol II (Guang et al, 2010); In addition, nuclear small RNAs are thought to recruit histone modifiers to the target’s chromatin, resulting in deposition of histone marks such as
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