H2S attenuates the myocardial fibrosis in diabetic rats through modulating PKC-ERK1/2MAPK signaling pathway.

Abstract

OBJECTIVETo investigate the roles and underlying mechanism of exogenous HS (hydrogen sulfide) in attenuating the myocardial fibrosis in diabetic rats.METHODS: A total of 40 SD rats were randomly divided into 4 groups: control group, STZ group, STZ HS group and HS group. To build the DM rat model , the rats in the STZ group and STZ HS group were injected streptozotocin (STZ) intraperitoneally, While the rats in the STZ HS group and the HS group received sodium hydrosulfide (NaHS), which provides exogenous HS. Eight weeks later, the myocardial tissues of rats were used to detecting the collagen deposition through Masson staining, as well as some protein expressions related to myocardial fibrosis and signaling pathway by western blotting.RESULTS: Comparing to control group, the collagen deposition of myocardial matrix remarkably increased in the STZ group, and almost all the proteins that are relative to myocardial fibrosis, inflammatory and signaling pathway show an overexpression, except for PPARG and NF-Bp65. When Compared with the STZ group, the collagen deposition was obviously attenuated in STZ HS group, as well as the protein expressions above-mentioned, While PPARG was up-regulated.CONCLUSION: The myocardial fibrosis in DM rats can be attenuated effectively by exogenous HS, and the underlying mechanism is likely to regulating PKC-ERK1/2MAPK signaling pathway, improving the MMPs/TIMPs expression dysregulation and inhibiting inflammatory reaction.