Abstract
The induction of glutathione S-transferase (GST, EC 2.5.1.18) with 2,3,5-triiodobenzoic acid (TIBA) in soybean ( Glycine max) hypocotyls was diminished by the natural antioxidants ascorbate and glutathione but not with 1,4-dithio-threitol (DTT). DTT, 1,2-benzenediol and 1,4-benzenediol induced GST activity in soybean hypocotyls. Furthermore, in the presence of luminol TIBA-treated soybean suspension cells exhibited strong biphasic chemiluminescence. The induced chemiluminescence was inhibited by added catalase (EC 1.11.1.6) and superoxide dismutase (EC 1.15.1.1) but not by the peroxidase inhibitor azide. These findings indicate that as a response to TIBA-treatment, soybean cells produce H 2O 2 and O 2− without involvement of peroxidase (EC 1.11.1.7). The time course of active oxygen species production was nearly identical to that described for cells treated with an incompatible pathogen.
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