H2 production from maltose derived from starch using the visible light, photosensitization of Mg chlorophyll-a from Spirulina

Abstract

A photoinduced H2 production system, coupling d-maltose degradation by glucoamylase and glucose dehydrogenase (GDH) and H2 production with colloidal platinum as a catalyst using the visible light-induced photosensitization of Mg chlorophyll-a (Mg Chl-a), has been developed. H2 production was continuous when the reaction mixture containing d-maltose, glucoamylase, GDH, NAD+, Mg Chl-a, Methyl Viologen (MV2+, an electron relay reagent) and colloidal platinum was irradiated by visible light. The amount of H2 production was estimated to be 5 ± 0.5 μmol after 4 h irradiation. The yield of the conversion of d-maltose to H2 in this system was ca. 1.8 %.