H. PYLORI STRAINS IN HOUSEHOLD MEMBERS OF CHILDREN WITH H. PYLORI INFECTION.

Abstract

26 H. pylori infection has been associated with low socioeconomic status and crowding conditions. H. pylori strains, Cag-A/ Vac-A, may be used to identify intra-familial contamination. Amis: To identify H. pylori strains, Cag-A/ Vac-A, within household members of symptomatic children who were histologically identified with H. Pylori infection. Methods: Serum samples of all household members of ten H. pylori seropositive children were prospectively collected. H. pylori seropositivity was identified by HM-CAP ELISA kit or FlexSure rapid test. H. pylori strains, Cag-A/ Vac-A, were identified by Immuno-probing Westerm blot (Helico blot 2.0; GeneLabs Diagnostics, S.A). Results: Ten families with a total of 32 household non-patient members were identified. 20 (62%) had experience non-specific abdominal symptoms in the past. 28 (88%) were seropositive for H. pylori infection. 12 (37%) were Cag-A positive, 9 (28%) were Vac-A positive, 8(25%) were positive for both strains, and 14 (44%) were negative for both strains. Identical H. pylori strain between patient and all household members was found in 4 (40%) families. In 4 families (40%), H. pylori strains identity was found in more than 50% of household members, and complete dissociation was found in 2 (20%) families. Conclusion: (1) H.pylori infection is commonly found within household members. (2) The significant identity of H. pylori strains in family members, suggest an intra familiar contamination. (3) To prevent re-contamination, members of household of children with H. pylori infection should be serologically screened.