Abstract
Mesophyll cells from Asparagus fern cladophylls were isolated quickly (3 h) and in large numbers (50 × 10 6) using a gentle grinding technique. A recording pH meter was used to show that isolated cells exposed to light or dark acidified the suspending medium at rates between 0.5 and 2.0 nmol H + per 10 6 cells/min. Acidification was inhibited within 3 min after addition of uncoupling agents, an inhibitor of the plasma membrane ATPase diethylstilbestrol (DES) or abscisic acid (ABA). It is suggested that acidification results from active H + excretion (or OH − uptake) and that these cells are a more suitable and convenient source of material for studies of H + excretion and related transport processes in leaf tissue than intact tissues or isolated protoplasts.
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