Gyrate atrophy of the choroid and retina: decreased ornithine aminotransferase concentration in cultured skin fibroblasts from patients

Abstract

A sensitive and convenient radioisotopic assay for ornithine aminotransferase (OAT) and an enzyme immunoassay for human ornithine aminotransferase were developed for studying decrease in activity of this enzyme in gyrate atrophy of the choroid and retina with hyperornithinemia. Picogram amounts of human OAT could be detected by the enzyme immunoassay. The OAT activity in cultured fibroblasts from two patients was 6% and 2% of that in control fibroblasts, and did not increase on addition of a high concentration of vitamin B 6. The decreased activity, assayed by enzyme immunoassay, was associated with decreased enzyme concentration, suggesting that deficiency of OAT in these patients is not due to production of a structurally altered enzyme lacking catalytic activity, but to decreased production of enzyme protein. When cells from patients were repeatedly subcultured, selective loss of both the activity and concentration of OAT was observed, without changes in activities of several other enzymes tested.