GYNOECIAL ONTOGENY AND MORPHOLOGY, AND POLLEN TUBE PATHWAY IN BLACK MAPLE, ACER SACCHARUM SSP. NIGRUM (ACERACEAE)

Abstract

The black maple (Acer saccharum Marsh, ssp. nigrum [Michx. f.] Desm.) gynoecium displays classical involute carpel development; carpels form, in mid‐ to late‐summer, as two separate, opposite, hood‐shaped primordia bearing naked megasporangia on inrolled carpel margins. Megasporogenesis, integument initiation, and carpel closure occur in spring; carpels fuse, forming a biloculate ovary with a short, hollow style and two divergent, dry, unicellular papillose stigmas. Transmitting tissues consist of developmentally and morphologically similar trichomes that form along the apparent carpel margins. The path from stigma to micropyle is open, but pollen tubes do not grow entirely ectotrophically. Germinating at the tip of a stigma papilla, a tube grows, apparently under the cuticle, to the papilla base. It then grows between stigma cells to the style, emerging to grow ectotrophically through the style to the compitum, where it passes into one of the locules. Within a locule, the tube grows over placenta and obturator to the micropyle, then between megasporangium cells to the female gametophyte, spreading over the surface near the egg. This study adds to our sparse understanding of gynoecium development and transmitting tissue in relation to pollen tube growth in naturally pollinated woody plants.