Gyne development in the Argentine ant Iridomyrmex humilis: role of overwintering and queen control

Abstract

Abstract A possible stimulatory effect of overwintering on gyne development in Iridomyrmex humilis (Mayr) was investigated. Although gyne‐potent larvae are present in the nest throughout the year, small queenless colony fragments composed of freshly overwintered ants (collected in late winter or early spring) produced 3–8 times more gynes than comparable fragments made up of non‐freshly overwintered ants (collected at other times of the year). Apparently, this stimulatory effect of overwintering acts on both the developmental potential of larvae and the tendency of workers to rear sexually competent larvae as gynes; queenless colony fragments in which either the brood or workers were freshly overwintered produced more gynes than fragments composed of non‐freshly overwintered workers or brood but fewer than fragments containing both brood and workers freshly overwintered. This increased sexualization potential of larvae due to overwintering is enough to overcome weak pheromonal inhibition of queens associated with low queen number; a single queen in a large freshly overwintered colony fragment is insufficient to inhibit gyne development, whereas ten queens are totally inhibitory. In non‐freshly overwintered colony fragments one queen is completely inhibitory. Variability in egg developmental potential according to queen age does not appear to play a major role in the seasonal production of gynes, because at least some eggs of very young queens (less than 3 weeks old) are capable of gyne development. In the field this stimulatory effect of overwintering is superimposed on a seasonal fluctuation in the combined strength of pheromonal queen control. In southern France, gynes are produced only in spring where they arise primarily from overwintered larvae just after a sharp drop in queen number, and presumably the total level of inhibitory queen pheromone, due to the massive execution of queens by workers.