Gut microbiota-dependent Trimethylamine N-Oxide are related with hip fracture in postmenopausal women: a matched case-control study.

Yakun Liu,Shaobin Jin,Yan-Long Guo,Shao-Guang Fan,Yang Li,Shan Meng,Hua Gao,Li-Juan Sui

doi:10.18632/aging.103283

Abstract

The study evaluates the serum levels of Trimethylamine N-Oxide (TMAO), a gut microbial metabolite, in 286 postmenopausal women with hip fracture. From January 1, 2018 to December 31, 2018, eligible patients were included. Same women without fracture mated age were enrolled. TMAO serum levels were tested by ultra-high-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS). The serum levels of TMAO were significantly higher in patients with hip fracture than in those controls (P<0.001). The serum levels of TMAO were also higher in patients with hip fracture only than in those who also had upper limb fracture (P=0.001). High level of TMAO was proved a predictor of both hip fracture and had upper limb fracture combined hip fracture, after the adjustment of other existing risk factors [e.g., for each 1 uM increase of TMAO, odd ratio 1.16 (95% CI, 1.07–1.25), P < 0.001; and 1.12 (95% CI, 1.03–1.26), P=0.008, respectively]. In summary, increased TMAO serum levels associated with high risk of hip fracture, suggesting that increase TMAO may contribute to osteoporosis and fracture in postmenopausal women.

Highlights

The incidences of osteoporosis and fractures is rising rapidly due to the aging society in China [1]
The Trimethylamine N-Oxide (TMAO) serum levels related to high-sensitivity C-reactive protein (r=0.212, P
The results showed that an increased risk of hip and upper limb fracture was associated with elevated TMAO

Summary

Introduction

The incidences of osteoporosis and fractures is rising rapidly due to the aging society in China [1]. Age is an independent risk factor for fracture and global aging brings urgency to fracture prevention [2]. Abnormal gut microbiota can affect the health of the elderly [3]. Previous studies showed that gut microbiota affected both digestive system and immune system, and associated with the osteoporosis and fractures [1]. The microbes are independent risk factors for obesity, diabetes, and osteoporosis [4]. Previous study had suggested that TMAO were associated with diabetes [6], metabolic syndrome [7], hypertension [8], stroke [9] and cardiovascular diseases [10]

Methods

Results

Conclusion