Abstract
BackgroundIrritable bowel syndrome (IBS) is common and difficult to treat and its pathogenesis is closely related to gut microbiota. However, differences in gut microbiota of patients in different regions make it more difficult to elucidate the mechanism of IBS. We performed an analysis of gut microbiota composition and functional prediction in Chinese patients with diarrhea-predominant IBS (IBS-D).MethodsFecal samples were obtained from 30 IBS-D patients and 30 healthy controls (HCs) in Nanchang, China. Using 16S gene sequence profiles, we analyzed the abundance of dominant microbiota at different taxonomy levels. Based on 16S information, Phylogenetic Investigation of Communities by Reconstruction of Unobserved States (PICRUSt) was used to predicting the function of gut microbiota.ResultsCompared to HCs, gut microbiota richness but not diversity was decreased in IBS-D patients. The abundant phyla Firmicutes, Fusobacteria and Actinobacteria decreased significantly, and Proteobacteria increased significantly in IBS-D patients. PICRUSt indicated that function expression of gut microbiota in IBS-D patients was up-regulated in metabolism of cofactors and vitamins, xenobiotics biodegradation and metabolism, and down-regulated in environmental adaptation, cell growth and death.ConclusionsCompared with the normal population in China, IBS-D patients are characterized by complex and unstable gut microbiota, which may influence inflammation and metabolism of the host.
Highlights
Irritable bowel syndrome (IBS) is common and difficult to treat and its pathogenesis is closely related to gut microbiota
Previous studies have found a large number of different kinds of intestinal flora in the human gut, but most of them were limited to the number and abundance of bacteria, which are only the tip of the iceberg compared to the function of these bacteria or the whole bacteria of nature
Through a case–control study, we aimed to address three questions related to gut microbiota in Diarrhea-predominant irritable bowel syndrome (IBS-D) patients and health population of Nanchang, China: What are the differences in the composition and abundance of gut microbiota between the two populations? In what ways does the function of these altered bacteria affect the host? What are the probable causes of these differences?
Summary
Irritable bowel syndrome (IBS) is common and difficult to treat and its pathogenesis is closely related to gut microbiota. Differences in gut microbiota of patients in different regions make it more difficult to elucidate the mechanism of IBS. Irritable bowel syndrome (IBS) is a common functional gastrointestinal (GI) disorder that affects 7–21% of the population worldwide [1] and 5–10% in most European countries, the US and China [2], but it differs depending on regions and diagnostic criterion. It causes significant socioeconomic burden on society [3]. More gut microbiota data of IBS patients from different regions are required
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