Abstract
Repeated stress-related diarrhea is a kind of functional bowel disorders (FBDs) that are mainly stemming from dysregulation of the microbiota–gut–brain axis mediated by a complex interplay of 5-hydroxytryptophan (5-HT). Intestinal content and intestinal mucosa microbiota belong to two different community systems, and the role of the two microbiota community systems in repeated stress-related diarrhea remains largely unknown. In order to ascertain the difference in composition and the potential function between intestinal content and intestinal mucosa microbiota response on repeated stress-related diarrhea, we collected intestinal contents and mucosa of mice with repeated stress-related diarrhea for 16S rRNA PacBio SMRT gene full-length sequencing, and with the digital modeling method of bacterial species abundance, the correlations among the two microbiota community systems and serum 5-HT concentration were analyzed. We found that the microbiotal composition differences both in intestinal contents and mucosa were consistent throughout all the phylogenetic ranks, with an increasing level of resolution. Compared with intestinal content microbiota, the diversity and composition of microbiota colonized in intestinal mucosa are more sensitive to repeated stress-related diarrhea. The PICRUSt2 of metagenomic function analysis found that repeated stress-related diarrhea is more likely to perturb the intestinal mucosa microbiota metagenomic functions involved in the neural response. We further found that the mucosal microbiota-based relative abundance model was more predictive on serum 5-HT concentration with the methods of machine-learning model established and multivariate dimensionality reduction (R2 = 0.876). These findings suggest that the intestinal mucosa microbiota might serve as a novel potential prediction model for the serum 5-HT concentration involvement in the repeated stress-related diarrhea, in addition to focusing on its mechanism in the gastrointestinal dysfunction.
Highlights
Psychosocial factors are associated with functional bowel disorders (FBDs) and might influence symptom severity (Black et al, 2020; Jones et al, 2020)
Bacterial DNA Sequences Are Divided by operational taxonomic units (OTUs) In an attempt to evaluate the effect of repeated stress-related diarrhea on intestinal microbiota, 10 week-old Kunming WT mice were treated with Folium senna extract combined with
Based on 16S rRNA PacBio SMRT gene full-length sequencing, we provide a comprehensive description of the biodiversity and taxonomic bacterial species composition of microbial communities of two regions, which respond to repeated stress-related diarrhea
Summary
Psychosocial factors are associated with functional bowel disorders (FBDs) and might influence symptom severity (Black et al, 2020; Jones et al, 2020). Functional diarrhea is more common in people with psychological comorbidity than in the rest of the general population, for its stemming from dysregulation of the gut– brain axis which is mediated by a complex interplay of intestinal microbiota imbalance and central nervous system processing (Chan et al, 2019; Black et al, 2020). The content of 5-HT in serum can represent the intestinal microbiota participating in the brain–gut nerve response of stress-related diarrhea to a certain extent. The research on the mechanism of psychological factors related to diarrhea is conducive to the development of drug therapy
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