Abstract
Gut acellular matrix for the in vitro study of Enteric Nervous System cells
Highlights
In the last decade, the acellular matrices obtained by a detergentenzymatic method [1], have been demonstrated to optimize in vitro and in vivo cell viability providing a near-physiologic environment
Acellular matrices (AMs) were stored at 4°C, in phosphate-buffered saline (PBS) supplemented with 1% antibiotic solution
In comparison to the native gut (Figure 1A), samples submitted to six cycles of decellularization showed to be lacking mucosal/ submucosal layers (Figure 1B)
Summary
The acellular matrices obtained by a detergentenzymatic method [1], have been demonstrated to optimize in vitro and in vivo cell viability providing a near-physiologic environment. Schrenk S (2019) Gut acellular matrix for the in vitro study of Enteric Nervous System cells homeostasis or the initiation and progression of pathologies. Under inflammatory bowel diseases (IBD) [1-5], dysfunctional motility, hypersensitivity and interruptions in the neuronal network are commonly observed and correlated to ECM disruption [6-8]. In this context, the development of a threedimensional cellular model could be beneficial to investigate ENSgut matrix interactions under in vivo-mimicking in vitro conditions. ENS cells are considered as limited to study in vitro the mechanisms underlining gut homeostasis or diseases
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