Guided adhesion and outgrowth of a constrained network on tailormade surfaces

Abstract

Event Abstract Back to Event Guided adhesion and outgrowth of a constrained network on tailormade surfaces Anke Wörz1*, Steffen Kandler2, Oswald Prucker1, Ulrich Egert3 and Jürgen Rühe1 1 Freiburg University, Department of Microsystems Engineering , Germany 2 Freiburg University, Neurobiology and Biophysics, Germany 3 Freiburg University, Department of Microsystems Engineering - Biomicrotechnology, Germany Planar micro-electrode arrays have now been used for some time to investigate the activity dynamics and response of neural networks to electrical or chemical stimuli. Constructing defined connectivity statistics under cell culture conditions that allow the maturation of the neuronal networks for a long period has not been achieved so far. Such networks would be useful to understand the influence of the composition, connectivity statistics, and plasticity in a neuronal network on the dynamics of electrical activity. Successful reports of constrained neuronal networks have used serum free medium to minimize the masking of the pattern by proteins. Serum free medium, however, impairs glia develop-ment, which has shown to have significant effects on network maturation and long-term vitality of the neurons, whereas serum containing medium may mask the patterned molecular layers (e.g. stamped proteins), which leads to a loss of constrained neuronal networks after several hours. The key issue is the control of the spatial and long-term stability of neuronal cell adhesion and neurite outgrowth by physicochemical surface modifications under cell culture conditions that allow long-term maintenance of these networks on a micro-scale. This can be achieved by using thin polymer coatings, whose cell adhesion property is not affected by serum-proteins. We report a simple way to tailor the surface-chemistry using a photochemical approach, leading to the covalent attachment of polymer layers to glass and MEA surfaces. We examined the biocompatibility of polymer layers with different properties, such as hydrophilicity and charge, and their potential to promote or inhibit neuronal cell adhesion. Finally, patterns with cell attractive domains were obtained using pin printing or -contact printing ( -CP) of polymer solutions. These patterns were able to guide neuronal cells in a constrained network and were stable over several weeks in a serum containing cell culture medium. Conference: Bernstein Symposium 2008, Munich, Germany, 8 Oct - 10 Oct, 2008. Presentation Type: Poster Presentation Topic: All Abstracts Citation: Wörz A, Kandler S, Prucker O, Egert U and Rühe J (2008). Guided adhesion and outgrowth of a constrained network on tailormade surfaces. Front. Comput. Neurosci. Conference Abstract: Bernstein Symposium 2008. doi: 10.3389/conf.neuro.10.2008.01.077 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 17 Nov 2008; Published Online: 17 Nov 2008. * Correspondence: Anke Wörz, Freiburg University, Department of Microsystems Engineering, Freiburg, Germany, woerz@imtek.de Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Anke Wörz Steffen Kandler Oswald Prucker Ulrich Egert Jürgen Rühe Google Anke Wörz Steffen Kandler Oswald Prucker Ulrich Egert Jürgen Rühe Google Scholar Anke Wörz Steffen Kandler Oswald Prucker Ulrich Egert Jürgen Rühe PubMed Anke Wörz Steffen Kandler Oswald Prucker Ulrich Egert Jürgen Rühe Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.