GTP and IAP shifts adenosine A 1-agonist binding in smooth muscle membranes to the low affinity state, similar to the one found in intact cells

Abstract

Abstract Adenosine A1 receptors in the smooth muscle cell line DDT1 MF-2 were characterized by radioligand binding using the antagonist [3H]-8-cyclopentyl-1,3-dipropylxanthine ([3H]DPCPX) as the ligand. Binding properties of adenosine agonists and antagonists to both intact cells and membranes were investigated.