Abstract
Our aim was to determine GSTT1 expression levels in left colon tumors and paired normal tissue in order to identify specific alterations in GSTT1 mRNA levels. Alterations in GSTT1 expression in twenty-four left- sided colon tumors and paired cancer free tissue were determined by qRT-PCR. Significant fold changes were determined with t-test. When compared with cancer free tissue, left colon cancers showed a significant decrease in GSTT1 expression. However, GSTT1 mRNA levels among different grades increased gradually in correlation with tumor grade. Our results suggest that downregulation of GSTT1 in left-sided colon cancers is an early event and is reversed with cancer progression, probably due to cellular defense mechanisms as a response to changes in the microenvironment.
Highlights
Colorectal cancer (CRC) is the third most common cancer for both genders worldwide (Ferlay et al, 2010)
Left colon cancers have shown a significant decrease in gluthatione S-transferase theta-1 (GSTT1) mRNA levels (Figure 1)
Our study demonstrates the downregulation of GSTT1 in left colon tumors
Summary
Colorectal cancer (CRC) is the third most common cancer for both genders worldwide (Ferlay et al, 2010). While some of the inter individual differences in CRC susceptibility can be attributed to sequence variations in xenobiotic metabolizing genes including gluthatione S-transferase theta-1 (GSTT1), altered expression levels as a result of epigenetic or transcriptional regulation effect colorectal cancer susceptibility. The shift in the balance of activation/deactivation of xenobiotics is a result of deregulated expression of various enzymes in addition to genomic variations resulting in non-functional proteins (Cotton et al, 2000). This process is important in digestive and excretory system tissues which are directly exposed to carcinogens
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