GSTP1andCYP1A1Gene Polymorphisms and Non-Hodgkin Lymphoma

Abstract

Objective: To determine the association between genetic variants of GSTP1 and CYP1A1 enzymes and individual susceptibility to diffuse large B-cell lymphoma (DLBCL), as well as correlations with tobacco smoking. Methods: Fifty patients with DLBCL and 50 apparently healthy volunteers were genotyped for detection of GSTP1 and CYP1A1 polymorphisms by the restriction fragment length polymorphism (RFLP) technique. Results: For GSTP1 313 A→G polymorphism, statistically significant differences were observed between the DLBCL and control groups for the AA, AG, and GG genotypes, as well as the G allele. For CYP1A1 4889 A→G (M2) polymorphism, statistically significant differences were observed for the AA and AG genotypes and the G allele. The relationship of smoking status to susceptibility to DLBCL in the presence of these genetic polymorphisms revealed that smoking status had no effect. Conclusion: GSTP1 313 A→G polymorphism is associated with a decreased risk of lymphoma, whereas CYP1A1 4889 A→G (M2) polymorphism is associated with an increased risk of lymphoma regardless of smoking status. * NHL : non-Hodgkin lymphoma ECOG : Eastern Cooperative Oncology Group LDH : lactate dehydrogenase SNPs : single nucleotide polymorphisms GST : glutathione S-transferase NADPH : nicotinamide adenine dinucleotide phosphate NQO1 : quinone oxidoreductase 1 DLBCL : diffuse large B-cell lymphoma IPI : International Prognostic Index RFLP : restriction fragment length polymorphism EDTA : ethylenediaminetetraacetic acid PCR : polymerase chain reaction dNTP : deoxyribonucleotide triphosphate CI : confidence interval CSF : cerebrospinal fluid NATs : N-acetyltransferases B-CLL : B-cell chronic lymphocytic leukemia CYPs : cytochrome P450s SLL : small lymphotic lymphoma CLL : chronic lymphocytic leukemia