Abstract
TCP transcription factors play important roles in diverse aspects of plant development as transcriptional activators or repressors. However, the functional mechanisms of TCPs are not well understood, especially in cotton fibers. Here, we identified a total of 37 non-redundant TCP proteins from the diploid cotton (Gossypium raimondii), which showed great diversity in the expression profile. GrTCP11, an ortholog of AtTCP11, was preferentially expressed in cotton anthers and during fiber initiation and secondary cell wall synthesis stages. Overexpression of GrTCP11 in Arabidopsis thaliana reduced root hair length and delayed flowering. It was found that GrTCP11 negatively regulated genes involved in jasmonic acid (JA) biosynthesis and response, such as AtLOX4, AtAOS, AtAOC1, AtAOC3, AtJAZ1, AtJAZ2, AtMYC2, and AtERF1, which resulted in a decrease in JA concentration in the overexpressed transgenic lines. As with the JA-deficient mutant dde2-2, the transgenic line 4-1 was insensitive to 50 μM methyl jasmonate, compared with the wild-type plants. The results suggest that GrTCP11 may be an important transcription factor for cotton fiber development, by negatively regulating JA biosynthesis and response.
Highlights
TCP transcription factors are plant-specific developmental regulators, named according to the first four characterized members of the family, namely maize (Zea mays) TEOSINTE BRANCHED1, snapdragon (Antirrhinum majus) CYCLOIDEA, and rice (Oryza sativa) PROLIFERATING CELL NUCLEAR ANTIGEN FACTOR1 and 2
A cotton class I TCP transcription factor GrTCP11 was characterized, which was homologous to AtTCP11
Heat-map and Quantitative reverse transcription polymerase chain reaction (qRT-PCR) analysis showed that GrTCP11 was preferentially expressed during the stages of fiber initiation and secondary cell wall (SCW) synthesis, FIGURE 6 | Relative expression level of the genes related to jasmonic acid (JA) biosynthesis and response in wild-type and GrTCP11-overexpressed transgenic lines (3-1, 4-1, 5-4, 8-3, and 10-4)
Summary
TCP transcription factors are plant-specific developmental regulators, named according to the first four characterized members of the family, namely maize (Zea mays) TEOSINTE BRANCHED1, snapdragon (Antirrhinum majus) CYCLOIDEA, and rice (Oryza sativa) PROLIFERATING CELL NUCLEAR ANTIGEN FACTOR1 and 2. The TCP transcription family can be categorized into two subfamilies, namely class I and class II, based on sequence similarities of the TCP domain, which act as activators or repressors by the formation of homodimers or heterodimers (Kosugi and Ohashi, 2002). They have been shown to be involved in a wide range of biological processes throughout the entire life span of plants. GhTCP12, GhTCP13, and GhTCP18 are functionally redundant in controlling branching
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