Growth, substrate consumption, and product formation kinetics of Phanerochaete chrysosporium and Schizophyllum commune mixed culture under solid-state fermentation of fruit peels.

Abstract

Kinetic analysis of solid-state fermentation (SSF) of fruit peels with Phanerochaete chrysosporium and Schizophyllum commune mixed culture was studied in flask and 7kg capacity reactor. Modified Monod kinetic model suggested by Haldane sufficiently described microbial growth with co-efficient of determination (R 2) reaching 0.908 at increased substrate concentration than the classical Monod model (R 2 = 0.932). Leudeking-Piret model adequately described product synthesis in non-growth-dependent manner (R 2 = 0.989), while substrate consumption by P. chrysosporium and S. commune fungal mixed culture was growth-dependent (R 2 = 0.938). Hanes-Woolf model sufficiently represented α-amylase and cellulase enzymes synthesis (R 2 = 0.911 and 0.988); α-amylase had enzyme maximum velocity (V max) of 25.19IU/gds/day and rate constant (K m) of 11.55IU/gds/day, while cellulase enzyme had V max of 3.05IU/gds/day and K m of 57.47IU/gds/day. Product yield in the reactor increased to 32.65mg/g/day compared with 28.15mg/g/day in shake flask. 2.5cm media thickness was adequate for product formation within a 6day SSF in the tray reactor.