Abstract
Guinea-pig epidermal cells were grown in vitro after release by gentle trypsin treatment. The cells formed clusters from which DNA-synthesizing cells grew to form confluent cell sheets. Treatment of the cultures with vitamin A acid (VAA) significantly increased cellular attachment in the first hours after seeding. This was followed by a steep rise in DNA synthesis as revealed by autoradiography and liquid scintillation counting. The increase was approximately 6-fold compared to the controls. Cellular out growth was considerably accelerated as shown by cell density counts. The results show that in vitro VAA exhibits potent mitogenic activity. Culturing of postembryonic epidermal cells seems to be greatly facilitated by the use of this compound.
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