Abstract
The effects of phosphatidic acid (PA) and lysophosphatidic acid (LPA) on the growth of Chinese hamster ovary (CHO) cells in serum-free media were evaluated by MTT assay. Supplementing PA or LPA to a protein-free basal synthetic medium or a serum-free medium containing insulin and transferrin markedly stimulated the growth of CHO cells, suggesting that PA and LPA are promising growth-promoting supplements for use in constituting a low-protein serum-free medium.
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