Abstract
Fusarium oxysporum Schlecht. grew and sporulated best at 30 °C and pH 7.5. Light was stimulatory to sporulation but inhibitory to growth. Among the carbon sources employed, sucrose supported maximum growth while sporulation was best on mannitol. The best nitrogen source for growth and sporulation was L-asparagine. During growth in liquid media containing pectin or a cellulosic carbon source, F. oxysporum released pectic and cellulolytic enzymes respectively into the medium. The cellulolytic enzymes were separated by molecular exclusion and ion exchange chromatography into three components of high molecular weight. One of these possessed the characteristic of a C 1 cellulase.
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