Growth, seed development and genetic analysis in wild type and Def mutant of Pisum sativum L

Kwadwo Owusu Ayeh,Anne Kathrine Hvoslef-Eide,Mike J Ambrose,YeonKyeong Lee

doi:10.1186/1756-0500-4-489

Kwadwo Owusu Ayeh, Anne Kathrine Hvoslef-Eide + Show 2 more

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https://doi.org/10.1186/1756-0500-4-489

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Abstract

BackgroundThe def mutant pea (Pisum sativum L) showed non-abscission of seeds from the funicule. Here we present data on seed development and growth pattern and their relationship in predicting this particular trait in wild type and mutant lines as well as the inheritance pattern of the def allele in F2 and F3 populations.FindingsPod length and seed fresh weight increase with fruit maturity and this may affect the abscission event in pea seeds. However, the seed position in either the distal and proximal ends of the pod did not show any difference. The growth factors of seed fresh weight (FW), width of funicles (WFN), seed width (SW) and seed height (SH) were highly correlated and their relationships were determined in both wild type and def mutant peas. The coefficient of determination R2 values for the relationship between WFN and FW, SW and SH and their various interactions were higher for the def dwarf type. Stepwise multiple regression analysis showed that variation of WFN was associated with SH and SW. Pearson's chi square analysis revealed that the inheritance and segregation of the Def locus in 3:1 ratio was significant in two F2 populations. Structural analysis of the F3 population was used to confirm the inheritance status of the Def locus in F2 heterozygote plants.ConclusionsThis study investigated the inheritance of the presence or absence of the Def allele, controlling the presence of an abscission zone (AZ) or an abscission-less zone (ALZ) forming in wild type and mutant lines respectively. The single major gene (Def) controlling this phenotype was monogenic and def mutants were characterized and controlled by the homozygous recessive def allele that showed no palisade layers in the hilum region of the seed coat.

Highlights

Introduction to population geneticsPearson Education, Inc, Upper saddle, river, NJ 07458 2004, 69-89.doi:10.1186/1756-0500-4-489 Cite this article as: Ayeh et al.: Growth, seed development and genetic analysis in wild type and Def mutant of Pisum sativum L
This study investigated the inheritance of the presence or absence of the Def allele, controlling the presence of an abscission zone (AZ) or an abscission-less zone (ALZ) forming in wild type and mutant lines respectively
In order to study whether seed fresh weight was a significant factor in pea seed abscission, we measured seed fresh weight at maximum size in each pod in all the four accessions used in this study

Summary

Introduction

Introduction to population geneticsPearson Education, Inc, Upper saddle, river, NJ 07458 2004, 69-89.doi:10.1186/1756-0500-4-489 Cite this article as: Ayeh et al.: Growth, seed development and genetic analysis in wild type and Def mutant of Pisum sativum L. The def mutant pea (Pisum sativum L) showed non-abscission of seeds from the funicule. A development funiculus (def) mutant pea (Pisum sativum) is known as a spontaneous mutation with monogenic recessive inheritance [1,2,3,4]. In wild type pea, there is a distinctive cell separation between funicle and seed coat that leads abscission of seed and results in detachment of seed from funicle. The wild type pea developed a double palisade layer and these may contribute to seed abscission [8]. The palisade layers were absent in the def mutant pea and the funicle remained firmly attached to the seed coat resulting in non-abscission of seed from the pod.

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