Abstract
Malaria is still a global health problem. Plasmodium is a single-cell protozoan parasite that causes malaria and is transmitted to humans through the female Anopheles mosquito. The previous study showed that Sonchus arvensis L. callus has antiplasmodial activity. Several treatments are needed for callus quality improvement for antimalarial compound production. This study aimed to examine the effect of dolomite [CaMg(CO3)2] on growth (morpho-anatomical structure and biomass), secondary metabolite production, and in vitro antiplasmodial activity of S. arvensis L. callus. In this study, leaf explants were grown in Murashige and Skoog medium with a combination of 2,4-dichlorophenoxyacetic acid (2,4-D, one mg/L) and 6-benzyl amino purine (BAP, 0.5 mg/L) with dolomite (50, 75, 100, 150, and 200 mg/L). The 21 days callus ethanolic and methanolic extract were analyzed by gas chromatography-mass spectrometry (GC-MS) and thin-layer chromatography (TLC). The antiplasmodial test was performed on a blood culture infected with Plasmodium falciparum strain 3D7 using the Rieckmann method. The results showed that dolomite significantly affected callus growth, metabolite profile, and in vitro antiplasmodial activity. Dolomite (150 mg/L) showed the highest biomass (0.590 ± 0.136 g fresh weight and 0.074 ± 0.008 g dry weight). GC-MS analysis detected four compounds from callus ethanolic extract. Pelargonic acid, decanoic acid, and hexadecanoic acid were major compounds. One new terpenoid compound is based on TLC analysis. S. arvensis L. callus has antiplasmodial activity with the IC50 value of 5.037 μg/mL. It was three times lower than leaf methanolic extract and five times lower than leaf ethanolic extract.
Highlights
Malaria is well known as the world’s primary disease, especially in the tropics
We evaluated the morpho-anatomy, growth, metabolite profiles, and antiplasmodial activity of S. arvensis L. callus
Sonchus arvensis L. callus under dolomite [CaMg(CO3)2] treatment and its in vitro antiplasmodial activity the dolomite stock was made by diluting powder with hydrochloric acid (HCl)
Summary
Malaria is well known as the world’s primary disease, especially in the tropics. It is endemic in Asian, African, and Latin American countries [1]. Millennium development goals target to stop the spread and decrease malaria incidence through indicators of reducing morbidity and mortality caused by malaria [2]. Malaria prevention efforts are significant, including malaria prevention, diagnosis, and treatment. Eighty percent of the world population still uses natural products for medicine, and 75% of patients with malaria use traditional medication to treat this disease [3]. Malaria medicine was derived from Chincona succirubra L., while a new malaria drug generation, artemisinin, was made from Artemisia annua L. Malaria medicine was derived from Chincona succirubra L., while a new malaria drug generation, artemisinin, was made from Artemisia annua L. [4]
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
