Abstract
As an important type of non-coding RNA molecule, long non-coding RNAs (lncRNAs) have varied roles in many biological processes, and have been studied extensively over the past few years. However, little is known about lncRNA-mediated regulation during cattle growth and development. Therefore, in the present study, RNA sequencing was used to determine the expression level of mRNAs and lncRNAs in the liver of adult Leiqiong cattle under the condition of growth retardation and normal growth. We totally detected 1,124 and 24 differentially expressed mRNAs and lncRNAs, respectively. The differentially expressed mRNAs were mainly associated with growth factor binding, protein K63-linked ubiquitination and cellular protein metabolic process; additionally, they were significantly enriched in the growth and development related pathways, including PPAR signaling pathway, vitamin B6 metabolism, glyoxylate and dicarboxylate metabolism. Combined analysis showed that the co-located differentially expressed lncRNA Lnc_002583 might positively influence the expression of the corresponding genes IFI44 and IFI44L, exerting co-regulative effects on Leiqiong cattle growth and development. Thus, we made the hypothesis that Lnc_002583, IFI44 and IFI44L might function synergistically to regulate the growth of Leiqiong cattle. This study provides a catalog of Leiqiong cattle liver mRNAs and lncRNAs, and will contribute to a better understanding of the molecular mechanism underlying growth regulataion.
Highlights
As an important type of non-coding RNA molecule, long non-coding RNAs have varied roles in many biological processes, and have been studied extensively over the past few years
It was suggested that daily feeding of 70 mg/kg body weight of cysteamine can significantly increase the daily weight gain and serum insulin like growth factor 1 (IGF1) levels of growing and finishing pigs, and can upregulate the expression of the growth hormone receptor (GHR), IGF1, IGF1 receptor (IGF1R), and insulin like growth factor binding protein 3 (IGFBP3) genes in multiple tissues[3]
89,186,385, 77,069,075, 80,073,002, 91,238,926, 94,484,701 and 89,487,657 mapped reads were obtained from the clean data from the GRC1, GRC2, GRC3, NGC4, NGC5, and NGC6 libraries, respectively, and more than 94% were mapped to the Bovine reference genome (Table 1)
Summary
As an important type of non-coding RNA molecule, long non-coding RNAs (lncRNAs) have varied roles in many biological processes, and have been studied extensively over the past few years. In the present study, RNA sequencing was used to determine the expression level of mRNAs and lncRNAs in the liver of adult Leiqiong cattle under the condition of growth retardation and normal growth. We focused on transcriptome analysis of the livers of growth retarded Leiqiong cattle and normal growth Leiqiong cattle to identify growthrelated mRNAs, lncRNAs, and regulatory pathways. We hypothesized that this approach would identify only the critical genes and pathways involved in the growth retardation response. The genes identified in this study could serve as candidates for breeding Leiqiong cattle with superior growth performance
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