Abstract
This study aims to understand the growth response of indigenous bacteria originating from oil sludge in Dumai which has the potential as Naphthalene and Fenantren degrading agents based on variations in substrate pH. The isolates used in this study were Isolate A, Isolate E, and Isolate F as a result of isolation from oil sludge in Dumai. Based on the results of the growth response test on various concentration in previous studies, we chose E isolates to be tested for its degradation ability. The bacterial growth response data was obtained based on OD measurements every 24 hours using a spectrophotometer and TPC at 72 hours using Nutrient Agar media. Based on these data, we determined the optimum pH for indigenous bacterial growth potential to degrade naphthalene or phenanthrene. In all variations of pH, bacterial isolate E experienced growth. This shows that bacterium E can use naphthalene and fenantren at normal pH range (pH 5-9).
Highlights
Dumai is one of the largest oil port and producing cities in Indonesia
Oil has an impact on environmental sustainability because oil sludge deposits as waste from refineries
This study aims to understand the growth response of indigenous bacteria originating from oil sludge in Dumai which has the potential as Naphthalene and Fenantren degrading agents based on variations in substrate pH
Summary
Dumai is one of the largest oil port and producing cities in Indonesia. oil has an impact on environmental sustainability because oil sludge deposits as waste from refineries. Oil sludge contains aliphatic, aromatic and poliaromatic hydrocarbons (PAHs) that are carcinogenic, mutagenic, and immuno-toxic, and have the potential to pollute the environment. The Environmental Protection Agency in the United States categorizes 16 types of PAHs as the main environmental polluters (Skupinska et al, 2004; Seo et al, 2012; Masakorala et al, 2013). Naphthalene and phenanthrene are types of PAHs that have two or three rings. Both can be degraded more than other PAHs (Kafilzadeh and Fatemeh, 2012; Stingley et al, 2004; Mallick and Dutta, 2008; Seo et al, 2012; Pawar et al, 2013)
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