Growth response of Bacillus coagulans on TiO2 nanoparticles by RNA-Seq analysis of the customary probiotic strain

Abstract

The phosphoenolpyruvate sugar phosphotransferase systems in bacteria are complex enzyme systems carrying out various important functions like detection, phosphorylation and transport of several sugar substrates including mono and disaccharides, amino sugars, etc. This key enzyme complex prevents the efflux of sugar substrates by phosphorylation and prepares them for energy production and metabolism. In this study, a high throughput RNA sequencing method was used to compare the gene expression in Bacillus coagulans treated with nano titanium dioxide (TiO2) rutile. Out of 3175 genes, 499 were differentially expressed. Gene Ontology analysis revealed 239 significant genes. In the biological process category, we found the phosphoenolpyruvate-dependent sugar phosphotransferase systems are the dominant ones and are involved in the uptake of specific carbohydrate sources. In our previous study, we showed increased growth of bacteria in presence of nano TiO2 anatase with a sharp rise in ATP concentrations. We know that, among metal oxide-based nanoparticles, TiO2 is placed at the topmost position in the agri-food sector based on its use and their reactivity with human beneficial gut flora, commonly known as probiotics, becoming a major concern. Hence, the interaction of TiO2 rutile nanoparticles on Bacillus coagulans was studied thoroughly at the transcriptomics level; with depth.