Abstract
Aspergillus flavus strains were isolated from locally available poultry feeds. Effect of temperature, pH and culture media on growth of Aspergillus flavus was studied. Temperature ranged from 4-42°C (4, 10, 20, 25, 30, 37 and 42°C) was examined. Except for 4°C and 10°C, the isolate was able to grow for the whole temperature range. The growth was maximum at 25°C and was influenced with increasing or decreasing of temperature from 42°C to 20°C.The lag time was strongly influenced by the temperature at lower temperature level than at higher temperature range. Effect of pH on growth of Aspergillus flavus was also examined; from comparison of 3 different pH levels, it is concluded that at most temperatures pH 6.5 showed a higher growth rate and as a consequence required a shorter time to achieve maximum colony diameter. No significant variations in the lag time were observed. A natural poultry feed meal agar medium (FMAM) was developed in the laboratory and growth of A. flavus was compared with other 2 synthetic dehydrated media namely; Czapeksdox Agar (CDA) and potato dextrose Agar (PDA). Poultry feed meal agar medium showed better growth response than Czapeksdox agar and potato dextrose agar at all conditions. At 25°C and pH 6.5 found optimum for growth of Aspergillus flavus in feed meal agar medium whereas, temperature 30°C and pH 6.5 found optimum for growth for Czapeksdox agar media and temperature 30°C and pH 6 showed high growth rate on potato dextrose agar. Poultry feed meal media showed high affinity for growth of mycelium and early spore formation than other media examined.Asian J. Med. Biol. Res. June 2016, 2(2): 221-228
Highlights
Food and feed usually become contaminated and spoiled by moulds during their production and especially during storage
Enriched cultures were inoculated on potato dextrose agar plates for colony development
The present study was carried out to investigate the effect of extrinsic factors namely temperature and pH on growth of Aspergillus flavus IMS1103 isolated from poultry feed and to evaluate the performance of a newly developed media (Feed Meal Agar Media) on growth and toxin production of Aspergillus spp
Summary
Food and feed usually become contaminated and spoiled by moulds during their production and especially during storage. In recent time, feed stuffs are contaminated by mould growths and especially presences of aflatoxins are reported frequently in poultry and fish feeds. For this reason, it is important to understand the growth characteristics and kinetics of these organisms at various environmental conditions and influences of extrinsic and intrinsic factors on their growth (Rosso and Robinson, 2001).Food losses resulting from occurrences of moulds are worldwide economic problem. Aspergillus flavus is a potential aflatoxin producer and infect some crops at field level and grows at post-harvest conditions.
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