Growth response and ion homeostasis in two bermudagrass (Cynodon dactylon) cultivars differing in salinity tolerance under salinity stress

Abstract

ABSTRACT Bermudagrass (Cynodon dactylon) is a salinity-tolerant turfgrass that has good use potential in the saline-alkali lands of warm regions. However, the systematic Na+ and K+ regulation mechanisms under salinity stress remain unclear at the whole plant level. Two bermudagrass cultivars differing in salinity tolerance were exposed to 0, 50, 100, 200, or 300 mM NaCl in a hydroponic system. Growth, absorption, transportation, and secretion of Na+ and K+, and gas exchange parameters were determined in both cultivars. K+ contents were decreased and Na+ contents and Na+/K+ ratios were increased in both bermudagrasses with increased salinity; however, lower Na+ content and Na+/K+ ratio and more stable K+ content were found in the leaves of the salinity-tolerant ‘Yangjiang’ than the salinity-sensitive ‘Nanjing’. Higher Na+ contents in root cortical cells were found than in the stele cells of ‘Yangjiang’, but the opposite was observed in ‘Nanjing’. Lower Na+ contents and higher K+ contents were found in vessels for ‘Yangjiang’ than for ‘Nanjing’. Salinity stress increased the selective transport of K+ over Na+ from roots to leaves and the Na+-selective secretion via salt glands, which were stronger in ‘Yangjiang’ than ‘Nanjing’. Net photosynthetic rate and stomatal conductance decreased in the two bermudagrasses with increased salinity; however, they were more stable in ‘Yangjiang’. The results suggested that bermudagrass could reduce Na+ accumulation and maintain K+ stability in leaves under salinity stress by restricting Na+ into vessels in roots, selectively transporting K+ over Na+ from roots to leaves, selectively secreting Na+ via leaf salt glands, and maintaining suitable stomatal conductance.