Abstract

Isolation, stock and in addition, monoxenic culture of specific species of Aspidiscidae, Aspidisca costata and Aspidisca lynceus were attempted successfully. Aspidiscidae are the typical protozoa found frequently in the activated sludge from municipal sewage treatment. Using a heterogeneous bacterial population isolated from the activated sludge as the protozoan food, the monoxenic growth rate (logarithmic growth phase) of Aspidiscidae and the effect of temperature on the growth rate were measured. The specific growth rate was 1·2–1·3 day −1 at 20°C, about one-half of that observed with Vorticellidae at the same temperature. Aspidiscidae examined were “stenophagic” and “horozoic”. The optimum temperature for growth was about 30°C and the value of activation energy for growth was ΔE = 13,800 cal mole −1.

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