Growth, productivity and nutrient removal rates of sea lettuce (Ulva lactuca) in a land-based IMTA system with white seabass (Atractoscion nobilis) in Southern California

Abstract

Three consecutive 3-week trials were conducted to assess the growth, productivity, and nutrient removal rates of sea lettuce (Ulva lactuca) cultured in effluents from white seabass (Atractoscion nobilis) raceways. All U. lactuca cultivation trials were conducted in 175 L tanks with a surface area of 0.89 m2 run in triplicate, with water quality and growth measurements taken weekly. For the shading trial, U. lactuca was stocked at 0.56 kg/m2 with either 0%, 30%, or 60% shade and seawater supplied at 63 tank volumes per day (vol./day) water exchange (7.6 L/min) for each tank. The removal rate of total ammonia nitrogen (TAN) decreased with increasing shade levels, and the peak exceeded 80% removal at 0% shading. Growth rate and productivity of U. lactuca under 0% shading was 12.09 ± 3.13%/d and 13.28 ± 4.66 g DW/m2/d, which were both significantly higher than other shading levels. For the stocking density and water exchange rate trial, the stocking density of U. lactuca was 0.56 or 1.12 kg/m2, and seawater exchange rates were 4, 12, or 63 vol./day in a 2-factor design. In this trial, TAN removal rates were 100% across all treatment combinations. Growth rate of U. lactuca was highest (20.36 ± 2.36%/d) at 0.56 kg/m2 under the exchange rate of 63 vol./day, and the productivity reached up to 30.89 ± 6.53 g DW/m2/d, which was not different than that of U. lactuca stocked with 1.12 kg/m2 at the same exchange rate (p = 0.47). In a trial comparing sand-filtered seawater (RAW seawater) with seawater effluent from a raceway containing A. nobilis (FISH seawater), TAN removal rate by U. lactuca was consistently nearly 100% when supplied with FISH seawater with a TAN concentration ranging from 0.11 to 0.18 mg/L. Growth rate and productivity of U. lactuca supplied with FISH seawater were 21.36 ± 2.25%/d and 33.83 ± 7.29 g DW/m2/d, respectively, which was not different than that for U. lactuca supplied with RAW seawater. However, the protein content of U. lactuca cultured with FISH seawater was significantly higher and C/N ratio significantly lower than those cultured with RAW seawater (p < 0.01), which indicated that U. lactuca was in a nitrogen-limited situation when cultured with RAW seawater. In light of the better understanding of U. lactuca performance achieved in our study, the optimum U. lactuca and A. nobilis density combinations will be confirmed in future studies based on nitrogen balance to maximize the nutrient removal rates and diversity of seafood production.