Abstract
Growth potentials of CCR5-tropic/CXCR4-tropic HIV-1mt clones in macaque cells
Highlights
Prototype HIV-1mt clones, CXCR4-tropic NL-DT5R, and dual-tropic (CXCR4- and CCR5-tropic) stHIV-1, have been generated by us (Kamada et al, 2006) and others (Hatziioannou et al, 2006), respectively
When examined in CD8+ cell-depleted pig-tailed macaque peripheral blood mononuclear cells (PBMCs), NL-DT5AD was found to be replication-competent in addition to NL-DT562 (Igarashi and Adachi, unpublished results)
To improve the replication ability of NL-DT562, we extensively modified its genome by adaptation to macaque cells and by in vitro mutagenesis (Nomaguchi et al, 2008, 2011, 2013a,b; Nomaguchi et al, submitted)
Summary
Prototype HIV-1mt clones, CXCR4-tropic NL-DT5R, and dual-tropic (CXCR4- and CCR5-tropic) stHIV-1, have been generated by us (Kamada et al, 2006) and others (Hatziioannou et al, 2006), respectively. We selected three distinct Env sequences and made three proviral constructs in the backbone of the NL-DT5R genome to obtain CCR5tropic/dual-tropic viruses (Figure 1A), based on the published results (Hsu et al, 2003; Hatziioannou et al, 2006; Matsuda et al, 2010; Nishimura et al, 2010).
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