Abstract
In a completely randomized design, 100 steers (269 kg) were allotted to 20 pens and fed a corn stalk/wheat middlings diet for 104 d. Treatments were no supplementation or supplementation with Aspergillus oryzae fermentation extract (AO) at 2 g/head/d. Feed intake, daily gain, and feed efficiency were unaffected by treatment (P>0.22). Ruminal fluid was collected with a vacuum pump via the esophagus near the end of the feeding period. Ruminal VFA and ammonia concentrations were similar across treatments (P>0.21) with the exception that the concentration of isobutyrate was greater (P=0.05) in steers supplemented with AO. Enumeration of ruminal bacteria showed greater counts of cellulolytic bacteria (P=0.07) in steers supplemented with AO. However, supplementation with AO did not affect ruminal fermentation end products or steer growth performance.
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