Abstract
Summary Growth parameters of a white spruce ( Picea glauca ) embryogenic suspension culture were characterized. Tissue fresh mass, dry mass, number of immature somatic embryos (stage 1), intracellular protein content, and total DNA accumulation showed similar growth curves over a 21-d culture period. Extracellular proteins gradually accumulated during the first 9 d, then dramatically increased. Immature embryo number showed a positive correlation to intracellular protein and DNA accumulation, correlation coefficients for these parameters were higher than for fresh and dry masses. The results from in vivo pulse-labelling with 35 S-methionine showed a peak for incorporation into proteins at day 7. The protein profiles of intracellular proteins were greatly different Erom those of extracellular proteins.
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