Abstract
Enumeration of yeast and molds on trypan blue (at 1:10 000 dilution) in potato dextrose sugar (trypan blue agar) was compared with potato dextrose agar (PDA) plus safranin flooding procedure using the hydrophobic grid-membrane filter technique (HGMF) of the ISO-GRID system. Yeast and mold grew well on trypan blue agar, producing easily recognized blue colonies on the ISO-GRID system. Results indicated that trypan blue agar is more convenient to use than the PDA plus safranin flooding procedure and as reliable (r = 0.99) for enumerating yeast and mold of cheeses, spices and dry products, and salad samples.
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