Abstract
Studies on the growth of batch suspension cultures of a plant cell line, initiated from Ipomoea sp. root explants, at temperatures ranging from 15 to 34 °C are reported. Maximum growth of cultures of this cell line occurred between 25 and 32 °C, with temperature variations within this range having little effect on growth rates based on dry cell yields. Cultivation at 20 °C substantially reduced the growth rate. However, cells grown at 20 °C failed to grow on transfer to fresh medium at 20 °C. Thus cultivation at suboptimum temperatures is not a satisfactory method for routine maintenance of the suspension culture.
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